fig5

Figure 5. In vitro and in vivo assessment of bioprinted BMP2-EVs (modified from^[105]). (A) Bioprinted patterns of Alexa Fluor 488-labeled BMP2 (green) loaded in PKH26-labeled EVs (red); (B) ALP staining of C2C12 cells at 72 h post-seeding on bioprinted BMP2-EVs patterns with indicated OPs; (C) Representative microCT 3D reconstructions of mouse leg scans containing either native EVs or BMP2-EVs bioprinted implants. Arrow points to heterotopic ossification; (D) Representative histological images showing H&E and Masson's trichrome staining of native EVs and BMP2-EVs bioprinted implants (*indicates bone tissue).