fig2

Figure 2. A: The AV-loop in the isolation chamber. V-femoral vein, A-femoral artery, G-venous graft, C-isolation chamber. After exposure of the femoral neurovascular bundle at the medial thigh of the rat, a femoral venous graft is harvested from the contralateral side and interposed between the femoral vessels by anastomoses. The isolation chamber is placed in the medial thigh of the rat and 300 µL of the fibrin matrix are applied at the bottom of the chamber. The arteriovenous fistula is laid onto the clot with the artery and vein exiting through an opening at the proximal pole and are covered with the rest of the fibrin clot. The lid is closed and the chamber with the matrix inside is fixed onto the adductor fascia at the medial thigh; B: after an interval of approximately 6 weeks, neovascular sprouts emerge from the AV-loop and form new fibrovascular tissue