fig4

Figure 4. The results of Western blot, pASK1 relative value and Bcl-2/Bax ratio in each group 72 h following ischemia-reperfusion. (a) Representative images of Western blots for pASK1, Bcl-2, and Bax; (b) pASK1 relative values in all groups. Compared to the ischemia-reperfusion group, the expression of pASK1 was significantly reduced in the hyperbaric oxygen, hydrogen-rich saline, and hyperbaric oxygen + hydrogen-rich saline groups; (c) the ratio between Bcl-2 and Bax in all groups. Bcl-2/Bax ratio reveals the highest level in hyperbaric oxygen + hydrogen-rich saline group. IR: ischemia reperfusion, HBO: postoperative hyperbaric oxygen, HRS: hydrogen-rich saline