fig2

Figure 2. The results of TdT-mediated dUTP-X nick end labeling staining and the apoptosis index 72 h following ischemia-reperfusion. (a) The evaluation of apoptotic cell death by TdT-mediated dUTP-X nick end labeling staining in all groups. Apoptotic cell number was reduced with hyperbaric oxygen preconditioning, hydrogen-rich saline and hydrogen-rich saline and hyperbaric oxygen preconditioning used cooperatively (brown staining indicates apoptotic cells [red arrow]; ×200); (b) the apoptosis index of all groups. The data shown indicate the percentage of TdT-mediated dUTP-X nick end labeling-positive cells and the total cell nuclei per field. Three different slide fields from different skin tissues were counted. TUNEL: TdT-mediated dUTP-X nick end labeling, IR: ischemia reperfusion, HBO: postoperative hyperbaric oxygen, HRS: hydrogen-rich saline